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generuler 100 bp plus dna ladder  (Thermo Fisher)


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    Structured Review

    Thermo Fisher generuler 100 bp plus dna ladder
    Generuler 100 Bp Plus Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/generuler+100+bp+plus+dna+ladder/100BP+DNA+LADDER/pmc12933600-119-12-18
    Average 97 stars, based on 1 article reviews
    generuler 100 bp plus dna ladder - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Detection, genetic characterization and reassortment analysis of Tribeč virus
    Article Snippet: .. Bold letters in the primer sequences are degenerated nucleotides. ( b ) Sensitivity of the RT-PCR tested with the prototype TRBV strain ( HQ266581 ): L – GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, Lithuania); 1–1.5 × 10 5 PFU/ml; 2–1.5 × 10 4 PFU/ml; 3–1.5 × 10 3 PFU/ml; 4–1.5 × 10 2 PFU/ml; 5–1.5 × 10 PFU/ml; 6–1.5 × 10 0 PFU/ml; - – negative control (water). ( c ) Specificity of the RT-PCR: L − 100 bp Plus DNA Ladder; 1 – prototype TRBV strain; 2 – Uukuniemi virus strain 37.C/2020/Dubrava/SVK (PX853966); 3 – TBEV strain Hypr (Ref-SKU: 008 N-EVA442); - – negative control (water). ( d ) DNA band of expected size in agarose gel after RT-PCR examination of ticks for orbiviruses: L – 100 bp Plus DNA Ladder; + – positive control (prototype TRBV strain); - – negative control (water); lines 1, 2, 3 and 5 – negative tick samples from this study; 4 – positive sample 16.C/2016/Dubrava/SVK. ( e ) The RT-PCR positive sample caused CPE in Vero E6 cells 3 days after infection (EC Plan-Neoflural 10×/0.3 Ph 1, Zeiss, Germany). ..

    Article Title: Detection, genetic characterization and reassortment analysis of Tribeč virus.
    Article Snippet: .. Bold letters in the primer sequences are degenerated nucleotides. (b) Sensitivity of the RT-PCR tested with the prototype TRBV strain (HQ266581): L – GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, Lithuania); 1–1.5 × 105 PFU/ml; 2–1.5 × 104 PFU/ml; 3–1.5 × 103 PFU/ml; 4–1.5 × 102 PFU/ml; 5–1.5 × 101 PFU/ml; 6–1.5 × 100 PFU/ml; - – negative control (water). (c) Specificity of the RT-PCR: L − 100 bp Plus DNA Ladder; 1 – prototype TRBV strain; 2 – Uukuniemi virus strain 37.C/2020/Dubrava/SVK (PX853966); 3 – TBEV strain Hypr (Ref- SKU: 008 N-EVA442); - – negative control (water). (d) DNA band of expected size in agarose gel after RT-PCR examination of ticks for orbiviruses: L – 100 bp Plus DNA Ladder; + – positive control (prototype TRBV strain); - – negative control (water); lines 1, 2, 3 and 5 – negative tick samples from this study; 4 – positive sample 16.C/2016/ Dubrava/SVK. (e) The RT-PCR positive sample caused CPE in Vero E6 cells 3 days after infection (EC Plan-Neoflural 10×/0.3 Ph 1, Zeiss, Germany). ..

    Negative Control:

    Article Title: Detection, genetic characterization and reassortment analysis of Tribeč virus
    Article Snippet: .. Bold letters in the primer sequences are degenerated nucleotides. ( b ) Sensitivity of the RT-PCR tested with the prototype TRBV strain ( HQ266581 ): L – GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, Lithuania); 1–1.5 × 10 5 PFU/ml; 2–1.5 × 10 4 PFU/ml; 3–1.5 × 10 3 PFU/ml; 4–1.5 × 10 2 PFU/ml; 5–1.5 × 10 PFU/ml; 6–1.5 × 10 0 PFU/ml; - – negative control (water). ( c ) Specificity of the RT-PCR: L − 100 bp Plus DNA Ladder; 1 – prototype TRBV strain; 2 – Uukuniemi virus strain 37.C/2020/Dubrava/SVK (PX853966); 3 – TBEV strain Hypr (Ref-SKU: 008 N-EVA442); - – negative control (water). ( d ) DNA band of expected size in agarose gel after RT-PCR examination of ticks for orbiviruses: L – 100 bp Plus DNA Ladder; + – positive control (prototype TRBV strain); - – negative control (water); lines 1, 2, 3 and 5 – negative tick samples from this study; 4 – positive sample 16.C/2016/Dubrava/SVK. ( e ) The RT-PCR positive sample caused CPE in Vero E6 cells 3 days after infection (EC Plan-Neoflural 10×/0.3 Ph 1, Zeiss, Germany). ..

    Article Title: Detection, genetic characterization and reassortment analysis of Tribeč virus.
    Article Snippet: .. Bold letters in the primer sequences are degenerated nucleotides. (b) Sensitivity of the RT-PCR tested with the prototype TRBV strain (HQ266581): L – GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, Lithuania); 1–1.5 × 105 PFU/ml; 2–1.5 × 104 PFU/ml; 3–1.5 × 103 PFU/ml; 4–1.5 × 102 PFU/ml; 5–1.5 × 101 PFU/ml; 6–1.5 × 100 PFU/ml; - – negative control (water). (c) Specificity of the RT-PCR: L − 100 bp Plus DNA Ladder; 1 – prototype TRBV strain; 2 – Uukuniemi virus strain 37.C/2020/Dubrava/SVK (PX853966); 3 – TBEV strain Hypr (Ref- SKU: 008 N-EVA442); - – negative control (water). (d) DNA band of expected size in agarose gel after RT-PCR examination of ticks for orbiviruses: L – 100 bp Plus DNA Ladder; + – positive control (prototype TRBV strain); - – negative control (water); lines 1, 2, 3 and 5 – negative tick samples from this study; 4 – positive sample 16.C/2016/ Dubrava/SVK. (e) The RT-PCR positive sample caused CPE in Vero E6 cells 3 days after infection (EC Plan-Neoflural 10×/0.3 Ph 1, Zeiss, Germany). ..

    Virus:

    Article Title: Detection, genetic characterization and reassortment analysis of Tribeč virus
    Article Snippet: .. Bold letters in the primer sequences are degenerated nucleotides. ( b ) Sensitivity of the RT-PCR tested with the prototype TRBV strain ( HQ266581 ): L – GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, Lithuania); 1–1.5 × 10 5 PFU/ml; 2–1.5 × 10 4 PFU/ml; 3–1.5 × 10 3 PFU/ml; 4–1.5 × 10 2 PFU/ml; 5–1.5 × 10 PFU/ml; 6–1.5 × 10 0 PFU/ml; - – negative control (water). ( c ) Specificity of the RT-PCR: L − 100 bp Plus DNA Ladder; 1 – prototype TRBV strain; 2 – Uukuniemi virus strain 37.C/2020/Dubrava/SVK (PX853966); 3 – TBEV strain Hypr (Ref-SKU: 008 N-EVA442); - – negative control (water). ( d ) DNA band of expected size in agarose gel after RT-PCR examination of ticks for orbiviruses: L – 100 bp Plus DNA Ladder; + – positive control (prototype TRBV strain); - – negative control (water); lines 1, 2, 3 and 5 – negative tick samples from this study; 4 – positive sample 16.C/2016/Dubrava/SVK. ( e ) The RT-PCR positive sample caused CPE in Vero E6 cells 3 days after infection (EC Plan-Neoflural 10×/0.3 Ph 1, Zeiss, Germany). ..

    Article Title: Detection, genetic characterization and reassortment analysis of Tribeč virus.
    Article Snippet: .. Bold letters in the primer sequences are degenerated nucleotides. (b) Sensitivity of the RT-PCR tested with the prototype TRBV strain (HQ266581): L – GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, Lithuania); 1–1.5 × 105 PFU/ml; 2–1.5 × 104 PFU/ml; 3–1.5 × 103 PFU/ml; 4–1.5 × 102 PFU/ml; 5–1.5 × 101 PFU/ml; 6–1.5 × 100 PFU/ml; - – negative control (water). (c) Specificity of the RT-PCR: L − 100 bp Plus DNA Ladder; 1 – prototype TRBV strain; 2 – Uukuniemi virus strain 37.C/2020/Dubrava/SVK (PX853966); 3 – TBEV strain Hypr (Ref- SKU: 008 N-EVA442); - – negative control (water). (d) DNA band of expected size in agarose gel after RT-PCR examination of ticks for orbiviruses: L – 100 bp Plus DNA Ladder; + – positive control (prototype TRBV strain); - – negative control (water); lines 1, 2, 3 and 5 – negative tick samples from this study; 4 – positive sample 16.C/2016/ Dubrava/SVK. (e) The RT-PCR positive sample caused CPE in Vero E6 cells 3 days after infection (EC Plan-Neoflural 10×/0.3 Ph 1, Zeiss, Germany). ..

    Agarose Gel Electrophoresis:

    Article Title: Detection, genetic characterization and reassortment analysis of Tribeč virus
    Article Snippet: .. Bold letters in the primer sequences are degenerated nucleotides. ( b ) Sensitivity of the RT-PCR tested with the prototype TRBV strain ( HQ266581 ): L – GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, Lithuania); 1–1.5 × 10 5 PFU/ml; 2–1.5 × 10 4 PFU/ml; 3–1.5 × 10 3 PFU/ml; 4–1.5 × 10 2 PFU/ml; 5–1.5 × 10 PFU/ml; 6–1.5 × 10 0 PFU/ml; - – negative control (water). ( c ) Specificity of the RT-PCR: L − 100 bp Plus DNA Ladder; 1 – prototype TRBV strain; 2 – Uukuniemi virus strain 37.C/2020/Dubrava/SVK (PX853966); 3 – TBEV strain Hypr (Ref-SKU: 008 N-EVA442); - – negative control (water). ( d ) DNA band of expected size in agarose gel after RT-PCR examination of ticks for orbiviruses: L – 100 bp Plus DNA Ladder; + – positive control (prototype TRBV strain); - – negative control (water); lines 1, 2, 3 and 5 – negative tick samples from this study; 4 – positive sample 16.C/2016/Dubrava/SVK. ( e ) The RT-PCR positive sample caused CPE in Vero E6 cells 3 days after infection (EC Plan-Neoflural 10×/0.3 Ph 1, Zeiss, Germany). ..

    Article Title: Detection, genetic characterization and reassortment analysis of Tribeč virus.
    Article Snippet: .. Bold letters in the primer sequences are degenerated nucleotides. (b) Sensitivity of the RT-PCR tested with the prototype TRBV strain (HQ266581): L – GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, Lithuania); 1–1.5 × 105 PFU/ml; 2–1.5 × 104 PFU/ml; 3–1.5 × 103 PFU/ml; 4–1.5 × 102 PFU/ml; 5–1.5 × 101 PFU/ml; 6–1.5 × 100 PFU/ml; - – negative control (water). (c) Specificity of the RT-PCR: L − 100 bp Plus DNA Ladder; 1 – prototype TRBV strain; 2 – Uukuniemi virus strain 37.C/2020/Dubrava/SVK (PX853966); 3 – TBEV strain Hypr (Ref- SKU: 008 N-EVA442); - – negative control (water). (d) DNA band of expected size in agarose gel after RT-PCR examination of ticks for orbiviruses: L – 100 bp Plus DNA Ladder; + – positive control (prototype TRBV strain); - – negative control (water); lines 1, 2, 3 and 5 – negative tick samples from this study; 4 – positive sample 16.C/2016/ Dubrava/SVK. (e) The RT-PCR positive sample caused CPE in Vero E6 cells 3 days after infection (EC Plan-Neoflural 10×/0.3 Ph 1, Zeiss, Germany). ..

    Article Title: A potent Vip3Aa94 protein from Thai Bacillus thuringiensis : molecular characterization and insecticidal potential
    Article Snippet: PCR amplification was performed using a T100TM Thermal Cycler (Bio-Rad) under the following conditions: initial denaturation at 96 °C for 4 min, followed by 35 cycles of denaturation at 96 °C for 40 s, annealing at 50 °C for 1 min, and extension at 72 °C for 2 min, with a final extension at 72 °C for 5 min. .. The resulting PCR products were analyzed by electrophoresis on a 1.5% agarose gel stained with RedSafe (iNtRON Biotechnology, Seongnam-si, South Korea) in 0.5X TAE buffer (0.02 M Tris base, 0.01 M acetic acid, 0.5 mM EDTA pH 8.0) at 100 V for 30 min. A GeneRuler 100 bp plus DNA ladder (Thermo Scientific) was used as a molecular size marker. .. The DNA bands were visualized using a Gel Doc system (Syngene).

    Article Title: Targeting STAT5A via CRISPR/Cas9 restores TKI sensitivity in resistant chronic myeloid leukemia cells.
    Article Snippet: .. PCR products were verified by electrophoresis on a 2% agarose gel at 100 V for 1 h. GeneRuler 100 bp Plus DNA Ladder (Thermo Fisher Scientific) was used to determine DNA size. ..

    Article Title: Isolation and Reassembly of Cultivable Bacteria and Yeasts for Kombucha Tea Fermentation
    Article Snippet: Glass beads were purchased from Sigma–Aldrich (St. Louis, MO, USA), all other chemicals and reagents were purchased from PanReac AppliChem (ITW Reagents, Monza, Italy). https://doi.org/10.3390/fermentation12020100 The quality and concentration of the extracted DNA were checked by NanoDropTM One spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). (GTG)5-rep-PCR was performed with primer GTG5 (5′-GTGGTGGTGGTGGTG-3′) according to Foti et al. [47]. .. Amplified PCR products were separated on a 2.0% w/v agarose gel for 2 h at 90 V. Amplicon size was determined using GeneRuler 100 bp Plus DNA Ladder (Invitrogen, Carlsbad, CA, USA). .. Gel images were captured using a ChemiDocTM MP Imaging System (BioRad Laboratories, Hercules, CA, USA).

    Positive Control:

    Article Title: Detection, genetic characterization and reassortment analysis of Tribeč virus
    Article Snippet: .. Bold letters in the primer sequences are degenerated nucleotides. ( b ) Sensitivity of the RT-PCR tested with the prototype TRBV strain ( HQ266581 ): L – GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, Lithuania); 1–1.5 × 10 5 PFU/ml; 2–1.5 × 10 4 PFU/ml; 3–1.5 × 10 3 PFU/ml; 4–1.5 × 10 2 PFU/ml; 5–1.5 × 10 PFU/ml; 6–1.5 × 10 0 PFU/ml; - – negative control (water). ( c ) Specificity of the RT-PCR: L − 100 bp Plus DNA Ladder; 1 – prototype TRBV strain; 2 – Uukuniemi virus strain 37.C/2020/Dubrava/SVK (PX853966); 3 – TBEV strain Hypr (Ref-SKU: 008 N-EVA442); - – negative control (water). ( d ) DNA band of expected size in agarose gel after RT-PCR examination of ticks for orbiviruses: L – 100 bp Plus DNA Ladder; + – positive control (prototype TRBV strain); - – negative control (water); lines 1, 2, 3 and 5 – negative tick samples from this study; 4 – positive sample 16.C/2016/Dubrava/SVK. ( e ) The RT-PCR positive sample caused CPE in Vero E6 cells 3 days after infection (EC Plan-Neoflural 10×/0.3 Ph 1, Zeiss, Germany). ..

    Article Title: Detection, genetic characterization and reassortment analysis of Tribeč virus.
    Article Snippet: .. Bold letters in the primer sequences are degenerated nucleotides. (b) Sensitivity of the RT-PCR tested with the prototype TRBV strain (HQ266581): L – GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, Lithuania); 1–1.5 × 105 PFU/ml; 2–1.5 × 104 PFU/ml; 3–1.5 × 103 PFU/ml; 4–1.5 × 102 PFU/ml; 5–1.5 × 101 PFU/ml; 6–1.5 × 100 PFU/ml; - – negative control (water). (c) Specificity of the RT-PCR: L − 100 bp Plus DNA Ladder; 1 – prototype TRBV strain; 2 – Uukuniemi virus strain 37.C/2020/Dubrava/SVK (PX853966); 3 – TBEV strain Hypr (Ref- SKU: 008 N-EVA442); - – negative control (water). (d) DNA band of expected size in agarose gel after RT-PCR examination of ticks for orbiviruses: L – 100 bp Plus DNA Ladder; + – positive control (prototype TRBV strain); - – negative control (water); lines 1, 2, 3 and 5 – negative tick samples from this study; 4 – positive sample 16.C/2016/ Dubrava/SVK. (e) The RT-PCR positive sample caused CPE in Vero E6 cells 3 days after infection (EC Plan-Neoflural 10×/0.3 Ph 1, Zeiss, Germany). ..

    Infection:

    Article Title: Detection, genetic characterization and reassortment analysis of Tribeč virus
    Article Snippet: .. Bold letters in the primer sequences are degenerated nucleotides. ( b ) Sensitivity of the RT-PCR tested with the prototype TRBV strain ( HQ266581 ): L – GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, Lithuania); 1–1.5 × 10 5 PFU/ml; 2–1.5 × 10 4 PFU/ml; 3–1.5 × 10 3 PFU/ml; 4–1.5 × 10 2 PFU/ml; 5–1.5 × 10 PFU/ml; 6–1.5 × 10 0 PFU/ml; - – negative control (water). ( c ) Specificity of the RT-PCR: L − 100 bp Plus DNA Ladder; 1 – prototype TRBV strain; 2 – Uukuniemi virus strain 37.C/2020/Dubrava/SVK (PX853966); 3 – TBEV strain Hypr (Ref-SKU: 008 N-EVA442); - – negative control (water). ( d ) DNA band of expected size in agarose gel after RT-PCR examination of ticks for orbiviruses: L – 100 bp Plus DNA Ladder; + – positive control (prototype TRBV strain); - – negative control (water); lines 1, 2, 3 and 5 – negative tick samples from this study; 4 – positive sample 16.C/2016/Dubrava/SVK. ( e ) The RT-PCR positive sample caused CPE in Vero E6 cells 3 days after infection (EC Plan-Neoflural 10×/0.3 Ph 1, Zeiss, Germany). ..

    Article Title: Detection, genetic characterization and reassortment analysis of Tribeč virus.
    Article Snippet: .. Bold letters in the primer sequences are degenerated nucleotides. (b) Sensitivity of the RT-PCR tested with the prototype TRBV strain (HQ266581): L – GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, Lithuania); 1–1.5 × 105 PFU/ml; 2–1.5 × 104 PFU/ml; 3–1.5 × 103 PFU/ml; 4–1.5 × 102 PFU/ml; 5–1.5 × 101 PFU/ml; 6–1.5 × 100 PFU/ml; - – negative control (water). (c) Specificity of the RT-PCR: L − 100 bp Plus DNA Ladder; 1 – prototype TRBV strain; 2 – Uukuniemi virus strain 37.C/2020/Dubrava/SVK (PX853966); 3 – TBEV strain Hypr (Ref- SKU: 008 N-EVA442); - – negative control (water). (d) DNA band of expected size in agarose gel after RT-PCR examination of ticks for orbiviruses: L – 100 bp Plus DNA Ladder; + – positive control (prototype TRBV strain); - – negative control (water); lines 1, 2, 3 and 5 – negative tick samples from this study; 4 – positive sample 16.C/2016/ Dubrava/SVK. (e) The RT-PCR positive sample caused CPE in Vero E6 cells 3 days after infection (EC Plan-Neoflural 10×/0.3 Ph 1, Zeiss, Germany). ..

    Polymerase Chain Reaction:

    Article Title: A potent Vip3Aa94 protein from Thai Bacillus thuringiensis : molecular characterization and insecticidal potential
    Article Snippet: PCR amplification was performed using a T100TM Thermal Cycler (Bio-Rad) under the following conditions: initial denaturation at 96 °C for 4 min, followed by 35 cycles of denaturation at 96 °C for 40 s, annealing at 50 °C for 1 min, and extension at 72 °C for 2 min, with a final extension at 72 °C for 5 min. .. The resulting PCR products were analyzed by electrophoresis on a 1.5% agarose gel stained with RedSafe (iNtRON Biotechnology, Seongnam-si, South Korea) in 0.5X TAE buffer (0.02 M Tris base, 0.01 M acetic acid, 0.5 mM EDTA pH 8.0) at 100 V for 30 min. A GeneRuler 100 bp plus DNA ladder (Thermo Scientific) was used as a molecular size marker. .. The DNA bands were visualized using a Gel Doc system (Syngene).

    Article Title: Characterization of antibiotic-resistant bacteria and phylogenetic analysis of E. coli strains isolated from healthy broilers in Rawalpindi, Pakistan
    Article Snippet: .. 12 μl PCR products were loaded in the gel along with a GeneRuler 100 bp Plus DNA ladder (Thermo Scientific) as a size marker and was run for 50 min at 110 V in a horizontal gel electrophoresis apparatus. .. The gel was visualized in a gel documentation system (Syngene) for the presence of desired products.

    Article Title: Targeting STAT5A via CRISPR/Cas9 restores TKI sensitivity in resistant chronic myeloid leukemia cells.
    Article Snippet: .. PCR products were verified by electrophoresis on a 2% agarose gel at 100 V for 1 h. GeneRuler 100 bp Plus DNA Ladder (Thermo Fisher Scientific) was used to determine DNA size. ..

    Article Title: Isolation and Reassembly of Cultivable Bacteria and Yeasts for Kombucha Tea Fermentation
    Article Snippet: Glass beads were purchased from Sigma–Aldrich (St. Louis, MO, USA), all other chemicals and reagents were purchased from PanReac AppliChem (ITW Reagents, Monza, Italy). https://doi.org/10.3390/fermentation12020100 The quality and concentration of the extracted DNA were checked by NanoDropTM One spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). (GTG)5-rep-PCR was performed with primer GTG5 (5′-GTGGTGGTGGTGGTG-3′) according to Foti et al. [47]. .. Amplified PCR products were separated on a 2.0% w/v agarose gel for 2 h at 90 V. Amplicon size was determined using GeneRuler 100 bp Plus DNA Ladder (Invitrogen, Carlsbad, CA, USA). .. Gel images were captured using a ChemiDocTM MP Imaging System (BioRad Laboratories, Hercules, CA, USA).

    Electrophoresis:

    Article Title: A potent Vip3Aa94 protein from Thai Bacillus thuringiensis : molecular characterization and insecticidal potential
    Article Snippet: PCR amplification was performed using a T100TM Thermal Cycler (Bio-Rad) under the following conditions: initial denaturation at 96 °C for 4 min, followed by 35 cycles of denaturation at 96 °C for 40 s, annealing at 50 °C for 1 min, and extension at 72 °C for 2 min, with a final extension at 72 °C for 5 min. .. The resulting PCR products were analyzed by electrophoresis on a 1.5% agarose gel stained with RedSafe (iNtRON Biotechnology, Seongnam-si, South Korea) in 0.5X TAE buffer (0.02 M Tris base, 0.01 M acetic acid, 0.5 mM EDTA pH 8.0) at 100 V for 30 min. A GeneRuler 100 bp plus DNA ladder (Thermo Scientific) was used as a molecular size marker. .. The DNA bands were visualized using a Gel Doc system (Syngene).

    Article Title: Targeting STAT5A via CRISPR/Cas9 restores TKI sensitivity in resistant chronic myeloid leukemia cells.
    Article Snippet: .. PCR products were verified by electrophoresis on a 2% agarose gel at 100 V for 1 h. GeneRuler 100 bp Plus DNA Ladder (Thermo Fisher Scientific) was used to determine DNA size. ..

    Staining:

    Article Title: A potent Vip3Aa94 protein from Thai Bacillus thuringiensis : molecular characterization and insecticidal potential
    Article Snippet: PCR amplification was performed using a T100TM Thermal Cycler (Bio-Rad) under the following conditions: initial denaturation at 96 °C for 4 min, followed by 35 cycles of denaturation at 96 °C for 40 s, annealing at 50 °C for 1 min, and extension at 72 °C for 2 min, with a final extension at 72 °C for 5 min. .. The resulting PCR products were analyzed by electrophoresis on a 1.5% agarose gel stained with RedSafe (iNtRON Biotechnology, Seongnam-si, South Korea) in 0.5X TAE buffer (0.02 M Tris base, 0.01 M acetic acid, 0.5 mM EDTA pH 8.0) at 100 V for 30 min. A GeneRuler 100 bp plus DNA ladder (Thermo Scientific) was used as a molecular size marker. .. The DNA bands were visualized using a Gel Doc system (Syngene).

    Marker:

    Article Title: A potent Vip3Aa94 protein from Thai Bacillus thuringiensis : molecular characterization and insecticidal potential
    Article Snippet: PCR amplification was performed using a T100TM Thermal Cycler (Bio-Rad) under the following conditions: initial denaturation at 96 °C for 4 min, followed by 35 cycles of denaturation at 96 °C for 40 s, annealing at 50 °C for 1 min, and extension at 72 °C for 2 min, with a final extension at 72 °C for 5 min. .. The resulting PCR products were analyzed by electrophoresis on a 1.5% agarose gel stained with RedSafe (iNtRON Biotechnology, Seongnam-si, South Korea) in 0.5X TAE buffer (0.02 M Tris base, 0.01 M acetic acid, 0.5 mM EDTA pH 8.0) at 100 V for 30 min. A GeneRuler 100 bp plus DNA ladder (Thermo Scientific) was used as a molecular size marker. .. The DNA bands were visualized using a Gel Doc system (Syngene).

    Article Title: Characterization of antibiotic-resistant bacteria and phylogenetic analysis of E. coli strains isolated from healthy broilers in Rawalpindi, Pakistan
    Article Snippet: .. 12 μl PCR products were loaded in the gel along with a GeneRuler 100 bp Plus DNA ladder (Thermo Scientific) as a size marker and was run for 50 min at 110 V in a horizontal gel electrophoresis apparatus. .. The gel was visualized in a gel documentation system (Syngene) for the presence of desired products.

    Molecular Weight:

    Article Title: Identification, safety evaluation and probiotic potential of lactic acid bacteria isolated from wheat.
    Article Snippet: Lactic acid bacteria (LAB) are vital to the food and health industries, yet their diversity and probiotic potential on wheat surfaces remain underexplored.. In this study, 199 Gram-positive and Catalase-negative bacterial strains were isolated and characterized from wheat grains and spikes, of which 45 were selected using RAPD-PCR and identified by 16S ribosomal DNA sequencing.. The phylogenetic analysis of the 16S ribosomal DNA sequences grouped the strains into eight species: Enterococcus faecium, Pediococcus acidilactici, Pediococcus pentosaceus, Enterococcus mundtii, Enterococcus durans, Lactiplantibacillus plantarum, Lacticaseibacillus casei, and Loigolactobacillus coryniformis.

    Nucleic Acid Electrophoresis:

    Article Title: Characterization of antibiotic-resistant bacteria and phylogenetic analysis of E. coli strains isolated from healthy broilers in Rawalpindi, Pakistan
    Article Snippet: .. 12 μl PCR products were loaded in the gel along with a GeneRuler 100 bp Plus DNA ladder (Thermo Scientific) as a size marker and was run for 50 min at 110 V in a horizontal gel electrophoresis apparatus. .. The gel was visualized in a gel documentation system (Syngene) for the presence of desired products.

    Amplification:

    Article Title: Isolation and Reassembly of Cultivable Bacteria and Yeasts for Kombucha Tea Fermentation
    Article Snippet: Glass beads were purchased from Sigma–Aldrich (St. Louis, MO, USA), all other chemicals and reagents were purchased from PanReac AppliChem (ITW Reagents, Monza, Italy). https://doi.org/10.3390/fermentation12020100 The quality and concentration of the extracted DNA were checked by NanoDropTM One spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). (GTG)5-rep-PCR was performed with primer GTG5 (5′-GTGGTGGTGGTGGTG-3′) according to Foti et al. [47]. .. Amplified PCR products were separated on a 2.0% w/v agarose gel for 2 h at 90 V. Amplicon size was determined using GeneRuler 100 bp Plus DNA Ladder (Invitrogen, Carlsbad, CA, USA). .. Gel images were captured using a ChemiDocTM MP Imaging System (BioRad Laboratories, Hercules, CA, USA).

    In Vivo:

    Article Title: Isolated nucleic acid binding domains
    Article Snippet: .. 500 ng of GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, MA, USA) in 10 μl of buffer (10 mM Tris-HCl, pH 8.0) was used as input DNA (Lane 1, FIG. 11A) and in vivo biotinylated nucleic acid binding domain immobilized on streptavidin-coated magnetic beads was used as the interaction partner. ..

    Binding Assay:

    Article Title: Isolated nucleic acid binding domains
    Article Snippet: .. 500 ng of GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, MA, USA) in 10 μl of buffer (10 mM Tris-HCl, pH 8.0) was used as input DNA (Lane 1, FIG. 11A) and in vivo biotinylated nucleic acid binding domain immobilized on streptavidin-coated magnetic beads was used as the interaction partner. ..

    Magnetic Beads:

    Article Title: Isolated nucleic acid binding domains
    Article Snippet: .. 500 ng of GeneRuler 100 bp Plus DNA Ladder (Thermo Scientific, MA, USA) in 10 μl of buffer (10 mM Tris-HCl, pH 8.0) was used as input DNA (Lane 1, FIG. 11A) and in vivo biotinylated nucleic acid binding domain immobilized on streptavidin-coated magnetic beads was used as the interaction partner. ..



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    Thermo Fisher generuler 100 bp plus ladder
    Generuler 100 Bp Plus Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/generuler+100+bp+plus+dna+ladder/DNA+Molecular+Weight+Marker%2C+100bp+Ladder/pm41543925-36-81-86
    Average 97 stars, based on 1 article reviews
    generuler 100 bp plus ladder - by Bioz Stars, 2026-09
    97/100 stars
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